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The Golgi Complex: Methods and Protocols by William J. Brown (English) Hardcover

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Item specifics

Condition
New: A new, unread, unused book in perfect condition with no missing or damaged pages. See the ...
ISBN-13
9781493964611
Type
NA
Publication Name
NA
ISBN
9781493964611
Publication Year
2016
Format
Hardcover
Language
English
Book Title
Golgi Complex : Methods and Protocols
Author
William J. Brown
Item Length
10in
Publisher
Springer New York
Genre
Science
Topic
Life Sciences / Cell Biology
Item Width
7in
Item Weight
206.5 Oz
Number of Pages
Xi, 219 Pages

About this product

Product Information

Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques to "in vitro" biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful "Methods in Molecular Biology" series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls. Practical and detailed, "The Golgi Complex: Methods and Protocols" will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.

Product Identifiers

Publisher
Springer New York
ISBN-10
1493964615
ISBN-13
9781493964611
eBay Product ID (ePID)
24038810296

Product Key Features

Book Title
Golgi Complex : Methods and Protocols
Author
William J. Brown
Format
Hardcover
Language
English
Topic
Life Sciences / Cell Biology
Publication Year
2016
Genre
Science
Number of Pages
Xi, 219 Pages

Dimensions

Item Length
10in
Item Width
7in
Item Weight
206.5 Oz

Additional Product Features

Series Volume Number
1496
Number of Volumes
1 Vol.
Lc Classification Number
Qh573-671
Table of Content
4D Confocal Imaging of Yeast Organelles.- Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons.- Imaging Golgi Outposts in Fixed and Living Neurons.- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor.- STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells.- Reconstitution of COPI Vesicle and Tubule Formation.- Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules.- Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures.- High Content Analysis of the Golgi Complex by Correlative Screening Microscopy.- Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo.- In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in AdherentMammalian Cell Cultures.- Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function.- Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway.- Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle.- The Role of Lysophospholipid Acyltransferases in the Golgi Complex.- Methods to Purify and Assay Secretory Pathway Kinases.
Copyright Date
2016
Lccn
2016-948830
Dewey Decimal
571.6/56
Intended Audience
Trade
Series
Methods in Molecular Biology Ser.
Dewey Edition
23
Illustrated
Yes

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Premier Books LLC
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